Overview
The present invention relates to an economical and efficient method for determining protein-protein interactions in plants by in vivo cross-linking of plant tissue using vacuum infiltration and chemical cross-linker to arrest the interacting proteins. The cytoplasmic interacting proteins are analyzed by comparing the protein profile using Sodium Dodecyl Sulphate- Polyacrylamide gel electrophoresis followed by Coomassie brilliant blue staining. The differences in the protein pattern demonstrate the interacting proteins in cytoplasm of plants due to the transduction of signals and other metabolic alterations.