Overview
The present invention relates to a novel thermostable peroxidase from aerial parts of Bael [Aegle marmelos (Linn.)Correa] and a method of isolation thereof. The thermostable peroxidase isoform is purified using heat fractionation of homogenate, ammonium sulphate precipitation and gel permeation chromatography. The enzyme catalyzes reactions with many phenolic substrates with visible colored product. It has ability to catalyze reaction in a temperature range from 20°C to 70°C with loss of less than 20 % activity. This thermostable peroxidase is a perfect agent to be used as marker enzyme for tagging the antibody to be used in ELISA based detection system.